Welcome Guest
Sign In
Register
Products
Popular Categories
Antibody Search
ELISA Kit Search
Biomolecules
Flow Cytometers
Thermal Cyclers
Microscopes
Transfection Reagents
Featured Products
New Products
Articles
Resources
eBooks
Bench Tips
Tech Insights
Editorial Articles
News
Product Reviews
Future Lab
Multimedia
Biocompare Webinars
Immunotherapy
Reproducibility
Transformative Technologies
Podcasts
Tech Talks
Bench Tip Videos
All Videos and Webinars
Life Science Webinars
Write a Review
Antibodies
Kits/Reagents
Equipment
Services
Resources
Promotions
Events
AACR SPOTLIGHT
Register
Sign In
Biocompare
Products
Cloning and Expression
Enzymes
NlaIII
Supplier Images
NlaIII
NlaIII from New England Biolabs
Be the first to write a review!
Citations:
(93)
Supplier Page
Supplier Page from
New England Biolabs for
NlaIII
Get Pricing
Product Specs
Company Info
Citations
Reviews
Protocols
Product Specs
Item
NlaIII
Company
New England Biolabs
Price
Pricing Info
Supplier Page
View Company Product Page
Catalog Number
R0125L
Quantity
2500 units
Concentration
10 u/µL
Applications
DNA Manipulation
Brand/Trademark
New England Biolabs Inc (NEB)
Type
Restriction Enzyme
Temperature
-80 C
Sequence
CATG/
Add to Compare List
New England Biolabs
240 County Road
Ipswich
,
Massachusetts
1938
United States
Phone
: 800-632-5227
800-632-7799
978-921-1350
Company Profile
Website:
http://www.neb.com
Citations (93)
(1)
DNA polymerase ε harmonizes topological states and R-loops formation to maintain genome integrity in Arabidopsis
Nature Communications
November 27, 2023
Qin Li, Jincong Zhou, Shuai Li, Weifeng Zhang, Yingxue Du, Kuan Li, Yingxiang Wang, Qianwen Sun
references34,35. In brief, 1 μg fragmented DNA (digested by
DdeI
(
R0175S
,
NEB
),
MseI
(
R0525S
,
NEB
),
NlaIII
(
R0125S
,
NEB
) and
MboI
(
R0147S
,
NEB
) endonucleases) was used for S9.6
immunoprecipitation
. Meanwhile, the same
(2)
DeSUMOylation of chromatin-bound proteins limits the rapid transcriptional reprogramming induced by daunorubicin in acute myeloid leukemias
Nucleic Acids Research
September 8, 2023
Mathias Boulanger, Mays Aqrouq, Denis Tempé, Chamseddine Kifagi, Marko Ristic, Dana Akl, Rawan Hallal, Aude Carusi, Ludovic Gabellier, Marion de Toledo,
et al.
pellets were resuspended in 700 μl of first enzyme manufacturer buffer 1X (
NlaIII
– cutsmart [
NEB
–
R0125L
]) and homogenized on ice (50
strokes
in total) with a 1 ml Dounce homogenizer. Cells were permeabilized
(3)
Differentiation block in acute myeloid leukemia regulated by intronic sequences of FTO
iScience
August 18, 2023
Francesco Camera, Isabel Romero-Camarero, Bradley H. Revell, Fabio M.R. Amaral, Oliver J. Sinclair, Fabrizio Simeoni, Daniel H. Wiseman, Lovorka Stojic, Tim C.P. Somervaille
Technologies,Cat#1081059
XbaI
,
NEB
,Cat#
R0145L
SbfI
,
NEB
,Cat#
R3642L
Age1,
NEB
,Cat#
R3552S
EcoR1-HF,
NEB
,Cat#
R3101L
DpnII
,
NEB
,Cat#
R0543S
NlaIII
,
NEB
,Cat#
R0125S
,, Critical commercial
assays
,Critical commercial
assays
,Critical commercial
assays
,, RNeasy Plus
(4)
Copy number variation alters local and global mutational tolerance
Genome Research
August 1, 2023
Grace Avecilla, Pieter Spealman, Julia Matthews, Elodie Caudal, Joseph Schacherer, David Gresham
sample, 2 μg of DNA was digested using
DpnII
(
NEB
R0543L
), and 2 μg of DNA was digested with
NlaIII
(
NEB
R0125L
). The reactions were heat inactivated, circularized by
ligation
using T4 Ligase (
Thermo Fisher Scientific
(5)
Multiomic analysis of cohesin reveals that ZBTB transcription factors contribute to chromatin interactions
Nucleic Acids Research
July 21, 2023
Rui Wang, Qiqin Xu, Chenlu Wang, Kai Tian, Hui Wang, Xiong Ji
temperature. The nuclei were isolated and treated with 25 μl of 10× cutsmart buffer and 100 U of
NlaIII
(
NEB
, #
R0125S
), and incubated overnight at 37°C, 900 rpm to digest the
chromatin
. The next day, the restriction enzyme
(6)
Contribution of the IGCR1 regulatory element and the 3′ Igh CTCF-binding elements to regulation of Igh V(D)J recombination
Proceedings of the National Academy of Sciences
June 27, 2023
Zhuoyi Liang, Lijuan Zhao, Adam Yongxin Ye, Sherry G. Lin, Yiwen Zhang, Chunguang Guo, Hai-Qiang Dai, Zhaoqing Ba, Frederick W. Alt
and
protease inhibitors
(
Roche
, #11836153001). Nuclei were digested with 700 units of
NlaIII
(
NEB
, #
R0125
) restriction enzyme at 37°C overnight, followed by
ligation
(
T4 DNA ligase
NEB
M0202L
) at 16°C overnight.
(7)
The genome-wide mutational consequences of DNA hypomethylation
Scientific Reports
April 27, 2023
Nicolle Besselink, Janneke Keijer, Carlo Vermeulen, Sander Boymans, Jeroen de Ridder, Arne van Hoeck, Edwin Cuppen, Ewart Kuijk
heat inactivation at 80 °C for 10 min. The genomic DNA was subsequently fragmented with 100 nl
NLAIII
(
R0125L
,
New England Biolabs
) in 1 × Cutsmart (New England Biolabs) for 2 h at 37 °C followed by heat inactivation
(8)
Elevated enhancer-oncogene contacts and higher oncogene expression levels by recurrent CTCF inactivating mutations in acute T cell leukemia
Cell Reports
April 14, 2023
Willem K. Smits, Carlo Vermeulen, Rico Hagelaar, Shunsuke Kimura, Eric M. Vroegindeweij, Jessica G.C.A.M. Buijs-Gladdines, Ellen van de Geer, Marjon J.A.M. Verstegen, Erik Splinter, Simon V. van Reijmersdal,
et al.
,
Beckman Coulter
,Cat#
A63880
Formaldehyde solution,Merck,Cat# 252549; CAS: 50–00-0
NlaIII
,
New England Biolabs
,Cat#
R0125S
T4 DNA ligase
,
New England Biolabs
,Cat#
M0202S
NspI
,
New England Biolabs
,Cat#
R0602S
TRIzol Reagent
,
Thermo Fisher
(9)
An autoimmune pleiotropic SNP modulates IRF5 alternative promoter usage through ZBTB3-mediated chromatin looping
Nature communications
March 3, 2023
Wang Z, Liang Q, Qian X, Hu B, Zheng Z, Wang J, Hu Y, Bao Z, Zhao K, Zhou Y,
et al
.
alcohol (25:24:1) (
ThermoFisher
,
15593-049
). The purified DNA samples were digested with
NlaIII
(
NEB
,
R0125L
) for the rs4728142-containing region (GRCh37/hg19 chr7: 128,573,714–128,574,338) or
CviQI
(
NEB
,
R0639L
)
(10)
T-REX17 is a transiently expressed non-coding RNA essential for human endoderm formation
eLife
January 31, 2023
Landshammer A, Bolondi A, Kretzmer H, Much C, Buschow R, Rose A, Wu HJ, Mackowiak SD, Braendl B, Giesselmann P,
et al
.
the Weintraub A.S. et al. protocol (Weintraub et al., 2017). Briefly,
NlaIII
(
New England Biolabs
,
R0125
) was used as the primary cutter and
DpnII
(
New England Biolabs
, R0543) as a secondary cutter. Touchdown
(11)
Strand asymmetry influences mismatch resolution during a single-strand annealing
Genome Biology
December 1, 2022
Victoria O. Pokusaeva, Aránzazu Rosado Diez, Lorena Espinar, Albert Torelló Pérez, Guillaume J. Filion
Inverse PCR
Ten micrograms of genomic DNA from
transfected
mESCs
were digested using 10 μl 10 U/μL
NlaIII
(
NEB
, #
R0125S
) in a 50 μL final volume for 3 h at 37 °C. The reaction was heat-inactivated at 65 °C for 20 min. The
(12)
Transposon insertional mutagenesis of diverse yeast strains suggests coordinated gene essentiality polymorphisms
Nature Communications
December 1, 2022
Piaopiao Chen, Agnès H. Michel, Jianzhi Zhang
Genomic DNA (2 × 2 μg) was separately digested with 50 units of
DpnII
(
NEB
#
R0543L
) and
NlaIII
(
NEB
#
R0125L
). Each of these four-cutter restriction enzymes has one cutting site approximately every 256 nucleotides,
(13)
Hi-TrAC reveals division of labor of transcription factors in organizing chromatin loops
Nat Commun
November 5, 2022
Shuai Liu, Yaqiang Cao, Kairong Cui, Qingsong Tang, Keji Zhao
elution buffer and digested with the restriction enzymes 2 μL
MluCI
(
NEB
,
R0538L
) and 2 μL
NlaIII
(
NEB
,
R0125L
) in 100 μL reaction mixture at 37 °C for 30 min. The reaction mixture was adjusted to 1x B&W buffer
(14)
EWS/FLI mediated reprogramming of 3D chromatin promotes an altered transcriptional state in Ewing sarcoma
Nucleic Acids Research
September 23, 2022
Iftekhar A Showpnil, Julia Selich-Anderson, Cenny Taslim, Megann A Boone, Jesse C Crow, Emily R Theisen, Stephen L Lessnick
Hutchinson
Cancer
Center, Seattle, WA, USA),
T4 DNA ligase
(
NEB
,
M0202
),
MboI
(
NEB
,
R0147
),
NlaIII
(
NEB
,
R0125
), Expand Long Template polymerase (
Roche
,
11759060001
),
RNAse A
(
ThermoFisher Scientific
,
AM2271
),
Proteinase K
(15)
Chromatin organizer SATB1 controls the cell identity of CD4+ CD8+ double-positive thymocytes by regulating the activity of super-enhancers
Nature Communications
September 22, 2022
Delong Feng, Yanhong Chen, Ranran Dai, Shasha Bian, Wei Xue, Yongchang Zhu, Zhaoqiang Li, Yiting Yang, Yan Zhang, Jiarui Zhang,
et al.
DNA were purified by ethanol precipitation. Secondary restriction was performed using
NlaIII
(
NEB
,
R0125L
). Secondary
ligation
was carried out with
T4 DNA Ligase
in diluted conditions. Ligated DNA products
(16)
Deciphering multi-way interactions in the human genome
Nature Communications
September 20, 2022
Gabrielle A. Dotson, Can Chen, Stephen Lindsly, Anthony Cicalo, Sam Dilworth, Charles Ryan, Sivakumar Jeyarajan, Walter Meixner, Cooper Stansbury, Joshua Pickard,
et al.
on ice. Permeabilized cells were then digested with a final concentration of 1 U/μL of
NlaIII
(
NEB
-
R0125L
) and brought to volume with nuclease-free water to achieve a final 1X digestion reaction buffer in 450
(17)
Loss-of-function mutation survey revealed that genes with background-dependent fitness are rare and functionally related in yeast
Proceedings of the National Academy of Sciences
September 13, 2022
Elodie Caudal, Anne Friedrich, Arthur Jallet, Marion Garin, Jing Hou, Joseph Schacherer
(
Invitrogen
). gDNA (2 × 2 µg) was digested in parallel with 50 U
DpnII
(
NEB
,
R0543L
) and
NlaIII
(
NEB
,
R0125L
) in 50 µL for 16 h at 37 °C. The enzymatic reactions were inactivated for 20 min at 65 °C and DNA fragments
(18)
The Role of Plasma Interleukin-6 Levels on Atherosclerotic Cardiovascular Disease and Cardiovascular Mortality Risk Scores in Javanese Patients with Chronic Kidney Disease
Journal of Personalized Medicine
July 10, 2022
Hendri Susilo, Mochammad Thaha, Budi Susetyo Pikir, Mochamad Yusuf Alsagaff, Satriyo Dwi Suryantoro, Citrawati Dyah Kencono Wungu, Nando Reza Pratama, Cennikon Pakpahan, Delvac Oceandy
final extension at 72 °C–5′.
PCR
products were then incubated with restriction enzyme NIaIII (Cat. No.
R0125S
,
New England Biolabs
, Ipswich, MA, USA) at 37 °C for 4 h [34]. The results of the
PCR-RFLP
showed 119
(19)
An optimized 4C-seq protocol based on cistrome and epigenome data in the mouse RAW264.7 macrophage cell line
STAR Protocols
June 1, 2022
Zhiqiang Huang, Cheng Wang, Eckardt Treuter, Rongrong Fan
proteins","Chemicals, peptides, and recombinant proteins" ,,
DpnII
,
NEB
,Cat#
R0543M
NlaIII
,
NEB
,Cat#
R0125L
1 kb DNA ladder
,
Thermo Fisher
,Cat#
10787018
Agarose,
Thermo Fisher
,Cat#
R0492
AMPure beads,
Beckman Coulter
(20)
The 3′ Pol II pausing at replication-dependent histone genes is regulated by Mediator through Cajal bodies’ association with histone locus bodies
Nature Communications
May 25, 2022
Hidefumi Suzuki, Ryota Abe, Miho Shimada, Tomonori Hirose, Hiroko Hirose, Keisuke Noguchi, Yoko Ike, Nanami Yasui, Kazuki Furugori, Yuki Yamaguchi,
et al.
HIST2H2AB, which are present on
chromosomes
1 and 6, respectively. For 4C-seq analysis, we used
NlaIII
(
R0125S
;
NEB
) and
DpnII
(
R0543S
;
NEB
) as RE1 and RE2, respectively. For the data analysis, pipe4C was used with
Page
<<
<
1
2
3
4
5
>
>>
Reviews
Be the First to Write a Review!
Protocols
Serial Analysis of Gene Expression (SAGE): Experimental Method and Data Analysis
October 01, 2007
NlaIII from New England Biolabs
Supplier Page
View Company Product Page
Pricing Info
Product Specs
Company Info
Citations
Reviews
Protocols
Back To Top